I wanted to do this experiment because I wanted to find out how clean our schools were. This is how I am doing this experiment. I ordered a test kit for bacteria on-line and waited to for it to come in. I am setting up my experiment using the Scientific Method.
Problem: What will grow the most bacteria...a school toilet seat, a school cafeteria table, a desk, or the water fountain spout?
Hypothesis: I believe that the toilet seat will grow the most bacteria because lots of people use it every day, and it's a bathroom, not the cleanest place in the world!
Materials: A bacteria growing kit, (I ordered mine from Science Bob). The packet included 6 large Petri dishes, 1 pack of laboratory grade nutrient agar power, 250 ml plastic beaker and 6 extra-long bacteria gathering cotton swabs. You also need a microwave, a refrigerator, a toilet, a cafeteria table, a desk and a water fountain.
Procedure:
1. Prepare the agar powder using directions included in the packet.
2. Go to the restroom of your local school, take the cotton swab and rub it on the toliet seat, be sure not to touch the cotton swab with your hands, this could contanimate your sample. Rub the cotton portion of the swab to expose as much of the cotton as possible. Do for at least 5 seconds for each sample. (You can label each swab with tape if you would like).
3. Repeat this procedure for the rest of the objects tested.
Please note if you don't see anything on the cotton swab, that doesn't mean that there is not bacteria on it!!!
4. After gathering each sample, put the sample on the agar surface. Lift the lid and run the cotton tip very gently across the sureface in a zig-zag pattern...be careful, it can break easily.
5. Tape the Petri dish shut and label it with information that might include the area tested and the date.
6. Allow the Perti dishes to sit undisturbed in a warm, dark place like a cabinet or closet.
7. Check the samples every few days to look at the growth and chart the progress.
Thank you very much for providing important information. All your information is very valuable to me.
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